primary antibodies against opg  (Bioss)

Journal: BMC Nephrology

Article Title: Role of OPG/RANKL/RANK/TLR4 signaling pathway in sepsis-associated acute kidney injury

doi: 10.1186/s12882-024-03648-1

Figure Lengend Snippet: The expression levels of OPG , RANKL , RANK , and TLR4 mRNA in kidney tissues. RT-qPCR detected the mRNA expression levels of each gene. ( A ) OPG mRNA. ( B ) RANKL mRNA. ( C ) RANK mRNA. ( D ) TLR4 mRNA. * P < 0.05, ** P < 0.01, **** P < 0.0001

Article Snippet: After electrophoresis separation, the proteins were transferred to the membrane, which was blocked with 5% skim milk for 2 h. Subsequently, the membrane was incubated overnight at 4 °C with the primary antibodies against OPG (1:1000; Affinity Bioscience, OH, USA), RANKL (1:3000; Proteintech Group, Inc, Wuhan, China), RANK (1:500; Abcam, Cambridge, UK), TLR4 (1:500; Proteintech Group, Inc), and β-actin (1:10000) (Tiandeyue (Beijing) Biotechnology Co., Ltd, China).

Techniques: Expressing, Quantitative RT-PCR

Journal: BMC Nephrology

Article Title: Role of OPG/RANKL/RANK/TLR4 signaling pathway in sepsis-associated acute kidney injury

doi: 10.1186/s12882-024-03648-1

Figure Lengend Snippet: The expression levels of OPG, RANKL, RANK, and TLR4 protein in kidney tissues. Western blot detected the protein expression levels in each group. ( A ) Representative Western blot results. ( B ) Relative level of OPG. ( C ) Relative level of RANKL. ( D ) Relative level of RANK. ( E ) Relative level of TLR4. * P < 0.05, ** P < 0.01, **** P < 0.0001

Article Snippet: After electrophoresis separation, the proteins were transferred to the membrane, which was blocked with 5% skim milk for 2 h. Subsequently, the membrane was incubated overnight at 4 °C with the primary antibodies against OPG (1:1000; Affinity Bioscience, OH, USA), RANKL (1:3000; Proteintech Group, Inc, Wuhan, China), RANK (1:500; Abcam, Cambridge, UK), TLR4 (1:500; Proteintech Group, Inc), and β-actin (1:10000) (Tiandeyue (Beijing) Biotechnology Co., Ltd, China).

Techniques: Expressing, Western Blot

Journal: BMC Nephrology

Article Title: Role of OPG/RANKL/RANK/TLR4 signaling pathway in sepsis-associated acute kidney injury

doi: 10.1186/s12882-024-03648-1

Figure Lengend Snippet: A schematic diagram illustrating the OPG/RANKL/RANK/TLR4 signaling pathway. RANKL competes with OPG for binding to RANK. Upon binding of RANKL to RANK, it triggers the interaction of RANK with TRAF6, leading to the suppression of the TLR4 signaling pathway, the activation of which is initiated upon the binding of LPS with CD14. This in turn inhibits the activation of the NF-κB signaling pathway and the subsequent release of inflammatory mediators

Article Snippet: After electrophoresis separation, the proteins were transferred to the membrane, which was blocked with 5% skim milk for 2 h. Subsequently, the membrane was incubated overnight at 4 °C with the primary antibodies against OPG (1:1000; Affinity Bioscience, OH, USA), RANKL (1:3000; Proteintech Group, Inc, Wuhan, China), RANK (1:500; Abcam, Cambridge, UK), TLR4 (1:500; Proteintech Group, Inc), and β-actin (1:10000) (Tiandeyue (Beijing) Biotechnology Co., Ltd, China).

Techniques: Binding Assay, Activation Assay